Performance of EAST in diagnosing inhalant allergens in children with allergic rhinitis

The performance of enzyme linked immunosorbent assays (EAST) for identifying six indoor allergens was evaluated using skin prick test (SPT) as reference tests in 154 children with allergic rhinitis. Sensitivity of EAST ranged from 9% (cat) to 54% (HDM) with specificity of 74%(cockroach) to 100% (cat) with an agreement ranged from 58 to 86%. Cut off values ?> ?0.35 kU/L showed best sensitivity and specificity. Our findings agree with extant literature which suggests that the ability of EAST to determine the precipitating allergen is moderate. Assays for definitively identifying the inhalant allergen are currently not available.     

腓肠肌腱膜瓣翻转联合阔筋膜移植治疗跟腱再断裂

目的:探讨腓肠肌腱膜瓣翻转联合阔筋膜移植修复跟腱再断裂的临床疗效。方法 :自2013年7月至2017年4月,对11例跟腱术后再断裂患者采用腓肠肌腱膜瓣翻转联合阔筋膜移植重建治疗,男10例,女1例;年龄25～48岁。所有患者术前患足跖屈力量减弱、单足提踵试验阳性。观察术后并发症,并于术后6个月采用美国足踝外科学会踝-后足功能评分(AOFAS)进行功能评价。结果:本组11例患者获得随访,时间6～11个月。所有患者手术切口均Ⅰ期愈合,无皮肤坏死、切口感染、深静脉血栓、再断裂等并发症。跟腱缺损长度4～7 cm。患者完全恢复负重时间8～11周。术后6个月AOFAS评分79～100分,其中优9例,良2例。结论:腓肠肌腱膜翻转联合阔筋膜移植修复跟腱再断裂,具有跟腱修复可靠,并发症少,跟腱功能恢复良好的优点,是一种有效的临床治疗方法。     

地奥司明片(葛泰)在颈椎病治疗的临床应用

目的 观察地奥司明片(葛泰)在门诊对颈椎病治疗的临床应用疗效.方法 单纯口服地奥司明片(葛泰)进行颈椎病治疗150例,进行临床疗效观察,采用甘露醇结合地塞米松治疗进行对照.结果 治疗1个疗程后,总有效率达80.67％ (121/150),显著效果48.67％ (73/150),起效快,服药期间未发现不良反应,治疗效果与甘露醇结合地塞米松治疗效果无差别.结论 地奥司明片(葛泰)治疗颈椎病可以起到明显的临床疗效,在门诊保守治疗的颈椎病患者中可以推荐使用.     

Zweymuller系统全髋关节置换术治疗髋臼发育不良的中期疗效分析

目的：评价Zweymuller螺旋臼结合髋臼加深技术治疗髋臼发育不良的中期疗效。方法：自1998年1月至2004年12月,采用Zweymuller系统进行全髋关节置换术治疗髋臼发育不良继发髋关节骨性关节炎患者56例62髋,男14例（15髋）,女42例（47髋）;平均年龄48.6岁（30～67岁）。术前所有患者均有髋关节疼痛和功能障碍。观察项目包括术后并发症、影像学及功能恢复情况。髋关节功能采用Harris评分标准进行评定。结果：56例获得随访,时间5～11年,平均6.5年。X线检查显示髋臼假体位于真臼位置,与周围骨床结合紧密,髋臼假体外展角35°～45°,股骨假体内、外翻3°以内,术后患肢短缩平均（0.5±0.2）cm。术后近期发生深静脉血栓20例,予溶栓治疗后好转。近期脱位1例,复位、制动3周后下地行走。4髋发生异位骨化、均为BrookⅡ型。无感染、神经损伤病例发生。术后Harris评分（87.4±3.5）分,与术前（43.2±6.7）分比较,差异有统计学意义（P〈0.01）。结论：Zweymuller螺旋臼结合髋臼加深技术治疗髋臼发育不良继发髋关节骨性关节炎中期疗效优良。     

Mitomycin C-polyethylene glycol controlled-release film inhibits collagen secretion and induces apoptosis of fibroblasts in the early wound of a postlaminectomy rat model

Background contextRecovery from spine surgery is usually accompanied with the development of epidural scar adhesions from the abnormal proliferation of fibroblast and extracellular matrix–related metabolism. Polyethylene glycol (PEG) could alleviate the postsurgical adhesion formation with still leaving a gap between the sheet and the dura. However, PEG film could not function as a three-dimensional barrier to prevent adhesion completely. Mitomycin C (MMC) could also reduce the scar formation after surgery, but cytotoxicity and the administrative pathway prevent its application.PurposeOur purpose was to design and attest the role of new delivery system MMC-PEG controlled-release film in preventing the epidural scar adhesions after laminectomy in the rat model.Study design/settingA total laminectomy of L1 in the rat model was used to assess epidural fibrosis between and around the spinal nerves using a histochemistry assessment along with flow cytometry analysis.Patient sampleThe sample comprises 64 adult male Sprague-Dawley rats.Outcome measuresThe outcome measures are macroscopic evaluation, histological analysis, and flow cytometry analysis.MethodsLumbar laminectomies at L1 and L2 with a L1–L2 disc injury were performed on 64 adult male Sprague-Dawley rats. The rats were then randomized into four groups. In Group I, 25 mg PEG film was applied on the dura mater in the laminectomy area before the layers were sutured. In Group II, a cotton pad soaked with 0.05% MMC solution was kept on the laminectomy area for 5 minutes. In Group III, 25 mg PEG film containing 0.01% MMC was implanted on the laminectomy area. In Group IV, the laminectomy area was flushed with saline before wound closure. The rats were sacrificed 4 weeks after the operation. Macroscopic evaluation and histological analysis of epidural scar adhesion with the hematoxylin and eosin stain and Masson stain were used followed by the quantification of hydroxyproline (Hyp) and flow cytometry analysis of the apoptosis of fibroblasts in the scar tissues.ResultsThe recovery of all rats was uneventful after the operations. In the laminectomy sites of rats treated with MMC or MMC-PEG, the dura mater was clean without any evident adhesion or membrane. Collagen tissue hyperplasia significantly decreased in the MMC- or MMC-PEG–treated models. Accordingly, Hyp concentration was significantly reduced in these two groups compared with saline-control group. In addition, the apoptosis of fibroblasts, however, was significantly elevated in the MMC or MMC-PEG group compared with the saline-control group.ConclusionsThese results demonstrate that the treatment of postlaminectomy wounds with MMC-PEG film reduces the severity of adhesion by decreasing the concentration of Hyp and increasing the apoptosis of fibroblasts.